BIOT 230 - Basic Immunology and Immunological Methods

(G only) CE

A brief survey of the components of the immune system and how they interact. B and T cell development, activation and culture, the role of cytokines, their production and purification, signal transduction processes in B-cell activation, the role of MHC complexes, immunoglobulin synthesis and origins of diversity, antigen-antibody interactions, practical aspects of raising and purifying polyclonal and monoclonal antibodies, handling and labeling of antibodies, applications of antibodies including Western blotting, ELISA, and immunohistochemistry. PREREQUISITE(S): BIOT 120 ; BIOT 200 or consent of department. Three hours lecture, three hours laboratory each week. Formerly BT 204.

4 semester hours

Course Outcomes:
Upon course completion, a student will be able to:

Explain and describe the characteristics of a mammalian immune system and how its components are utilized in biotechnology
Complete routine laboratory tasks common to an immunology laboratory such as buffer preparation, dilutions, protein assays, protein gel electrophoresis, purification of antibodies, Western Blotting, and ELISAs
Complete calculations that are routinely encountered in an immunology lab such as buffer preparation, dilutions, protein concentrations, standard curves and unknown concentrations, and purification factors
Record, analyze, and evaluate data routinely collected in a protein laboratory

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