Montgomery College 2019-2020 Catalog 
    Oct 28, 2020  
Montgomery College 2019-2020 Catalog [ARCHIVED CATALOG]

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BIOT 230 - Basic Immunology and Immunological Methods

(G only)

A brief survey of the components of the immune system and how they interact. B and T cell development, activation and culture, the role of cytokines, their production and purification, signal transduction processes in B-cell activation, the role of MHC complexes, immunoglobulin synthesis and origins of diversity, antigen-antibody interactions, practical aspects of raising and purifying polyclonal and monoclonal antibodies, handling and labeling of antibodies, applications of antibodies including Western blotting, ELISA, and immunohistochemistry. PREREQUISITE(S): BIOT 200  or consent of department. Three hours lecture, three hours laboratory each week. Formerly BT 204.

4 semester hours

Course Outcomes:
Upon course completion, a student will be able to:

  • Explain and describe the characteristics of a mammalian immune system and how its components are utilized in biotechnology
  • Complete routine laboratory tasks common to an immunology laboratory such as buffer preparation, dilutions, protein assays, protein gel electrophoresis, purification of antibodies, Western Blotting, and ELISAs
  • Complete calculations that are routinely encountered in an immunology lab such as buffer preparation, dilutions, protein concentrations, standard curves and unknown concentrations, and purification factors
  • Record, analyze, and evaluate data routinely collected in a protein laboratory

Click here for the Fall 2020 Class Schedule

Click here for the Extended Winter 2021 Class Schedule

Click here for the Winter 2021 Class Schedule

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