BIOT 231 - Immunological Methods

(G only)

Brief survey of the most common immunoassay techniques used to detect biomarkers qualitatively and quantitatively. Sample preparation and buffers for immunoassays. Handling of antibodies such as reconstitution and dilution of primary and secondary antibodies. Determination of antibody specificity. Use of multichannel pipettes, multiwell plates and plate readers. Western blotting, ELISA and immunohistochemistry. Bench top purification of polyclonal or monoclonal antibodies using protein A chromatography. Preparation of an alum absorbed immunogen. PREREQUISITE(S): BIOT 201 PRE- or COREQUISITE(S): BIOT 230 or consent of department. Three hours laboratory each week.

1 semester hour

Course Outcomes:
Upon course completion, a student will be able to:

Follow SOPs and GLP principles as they relate to immunoassays.
Complete routine laboratory tasks common to an immunology laboratory such as buffer preparation, dilutions, tissue homogenization, protein assays, protein gel electrophoresis, western blotting, ELISAs, antibody reconstitution and dilution, gel and blot documentation.
Record, analyze, and evaluate data routinely collected in an immunoassay laboratory.

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