BIOT 120 - Introduction to Cell Culture

An introduction to the basic principles of cell culture. Classification and origin of cells in culture, growth curves and associated calculations, cell culture laboratory equipment, causes of contamination, detection of contamination, environmental monitoring, prevention of contamination, cell line authentication. Cell culture growth environment incubators, atmosphere, buffers, media and media supplements. Experimental design with an emphasis on root cause analysis. Assessment Level(s): ENGL 101 /ENGL 011 , MATH 050 , READ 120 or higher. Two hours lecture.

2 semester hours

May not be taken concurrently with MATH 017 or MATH 020 or MATH 030 or MATH 045 without appropriate Math assessment score.

Course Outcomes:
Upon course completion, a student will be able to:

Describe the characteristics of mammalian cells grown in vitro.
Explain routine laboratory tasks in a cell culture laboratory such as aseptic work, passaging, feeding, and counting cells.
Complete commonly used calculations in cell culture such as media preparation, cell counting, and cell concentration dilutions.
Understand the basic concepts of experimental design in the context of cell culture.
Analyze and evaluate data related to the growth, maintenance, and evaluation of cell cultures.

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