BIOT 121 - Aseptic Technique and Cell Culture Skills

(G only)

An introduction to fundamental laboratory methods required for the successful growth of both anchorage dependent and suspension animal cells in culture. The emphasis is on Good Laboratory Practice (GLP) as applied to animal cell culture. How to read and use SOPs including traceability. Proper gowning. Proper aseptic technique and use of the biological safety cabinet. Preparation of media, feeding and passaging of cells, and initial scale-up. Cell counting, including viability determination both manually and semi-automated and seeding at a specific concentration. Environmental monitoring and contamination testing. PRE- or COREQUISITE(S): BIOT 120. Three hours laboratory each week.

1 semester hour

Course Outcomes:
Upon completion of this course, a student will be able to:

Demonstrate proper gowning and aseptic technique.
Perform routine laboratory tasks in a cell culture laboratory such as passaging, feeding, and counting cells.
Follow SOPs and perform GXP documentation correctly.
Conduct basic environmental monitoring and contamination detection.

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