BIOT 200 - Protein Biotechnology

An introduction to protein structure and function. Primary, secondary, tertiary, and quaternary structure are discussed in relation to protein function and stability. Causes of protein denaturation and its prevention by formulations for biologics. Functional assays for proteins such as enzymes and receptors are described in terms of experimental design, data collection, and data analysis. Strategies and methods of protein purification and quality determination such as chromatographic, electrophoretic and spectrophotometric techniques, peptide mapping and sequencing are presented. Relevant calculations such as buffer and solution preparation, standard curves, and kinetic assays will be discussed. PREREQUISITE(S): BIOL 150 or CHEM 131 or consent of the department. Three hours lecture.

3 semester hours

Course Outcomes:
Upon course completion, a student will be able to:

Explain the relationship between protein structure and function as they apply to biotechnology, especially in a drug discovery and therapeutic context.
Describe the basic principles of a functional protein assay.
Explain the basics of protein purification using chromatography.
Explain the analysis of proteins using electrophoretic and spectrophotometric methods.
Complete calculations that are routinely encountered in a protein lab such as buffer preparation, dilutions, protein concentrations and standard curves, enzyme activity, and specific activities.

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