BIOT 120 - Cell Culture and Cell Function

(G only)

An introduction to fundamental methods used to grow animal cells in culture and associated principles of cell structure and function. Topics in this course include aseptic technique, preparation and use of various culture media, cell counting and dilution, maintenance and propagation of cell lines, origin and uses of various cell lines, contamination, cell staining techniques, and quality control. A survey of metabolism, cell structure and function, growth factors and signal transduction. Assessment Level(s): ENGL 101 /ENGL 101A , MATH 093 /MATH 096 , READ 120 or higher. Two hours lecture, three hours laboratory each week. Formerly BT 117.

3 semester hours

Course Outcomes:
Upon course completion, a student will be able to:

Describe and explain the characteristics of mammalians cells grown in vitro
Perform routine laboratory tasks in a cell culture laboratory such as cell counting, cell passaging, and cell feeding
Complete commonly used calculations in cell culture such as media preparation, cell counting, and cell concentration dilutions and use the results in a laboratory setting
Record, analyze, and evaluate data related to the growth, maintenance, and evaluation of cell cultures

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