BIOT 200 - Protein Biotechnology

(G only)

This course provides an introduction to protein structure and function. Topics include primary, secondary, tertiary, and quarternary structure. Peptide and protein synthesis and translation systems for protein production are considered along with preservation of structure/function. Functional assays for proteins including basic principles of enzymology, enzyme kinetics, and binding assays are discussed. Strategies and methods of protein purification are considered with emphasis on chromatographic and electrophoretic techniques. Principles of proteomics including peptide mapping and sequencing. Diagnostic, therapeutic, and industrial applications of protein products are discussed. PREREQUISITE(S): BIOL 150 or CHEM 131 or consent of the department. Three hours lecture, three hours laboratory each week. Formerly BT 200.

4 semester hours

Course Outcomes:
Upon course completion, a student will be able to:

Explain and describe the characteristics of protein structure and function as they apply to biotechnology, especially in a drug discovery and therapeutic context
Complete laboratory techniques that are commonly used in protein laboratories such as buffer preparations, dilutions, protein assays, protein gel electrophoresis, functional protein assays, and basic protein purification
Complete calculations that are routinely encountered in a protein lab such as buffer preparation, dilutions, protein concentrations and standard curves, enzyme activity, and specific activities
Record, analyze, and evaluate data routinely collected in a protein laboratory

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