Montgomery College 2022-2023 Catalog 
    Mar 21, 2023  
Montgomery College 2022-2023 Catalog
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BIOT 230 - Applied Immunology

Brief survey of the critical, biotechnology relevant components of the innate and specific immune system including immune cells, cytokines, and antibodies. A survey of the related signal transduction systems such as JAK STAT and NfκB pathways. A brief description of immunotherapies such as Dendritic cell vaccines, CAR-T, immune checkpoint therapies monoclonal antibodies, and alternate antibody formats such as BiTEs and DARTs. Antigen antibody interactions, epitope mapping, and measurement of binding affinity. Principles of immunoassays with an emphasis on Western Blotting, ELISAs and Immunohistochemistry. Practical aspects such antibody labeling, antibody handling, storage and dilution, analysis of immunoassay data. PREREQUISITE(S): BIOT 200  or consent of department. Three hours lecture.

3 semester hours

Course Outcomes:
Upon course completion, a student will be able to:

  • Describe the components of the complete immune response and the role of key cells and molecules.
  • Follow and explain a signal transduction pathway involved in modulating the immune response.
  • Explain various immunotherapies and how they are produced, what they treat, and the benefits and risks of each.
  • List the key components of an immunoassay, critical considerations in designing an immunoassay, and the requirements for it to be valid.
  • Complete routine calculations related to immunoassays such as standard curves, serial dilutions, and solution preparation.

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