BIOT 201 - Protein Biotechnology Skills

(G only)

Provides an introduction to the skills needed to assess protein structure and function, such as protein quantitation assays and standard curves. Solution and buffer preparation, micropipetting, and use of standard equipment such as centrifuges and spectrophotometers. SDS-PAGE, staining, and documentation of gels. Strategies and methods of protein purification are considered along with emphasis on chromatographic and electrophoretic techniques. An emphasis on peptide mapping and sequencing is included. PRE- or COREQUISITE(S): BIOT 200 or consent of department. Three hours laboratory each week.

1 semester hour

Course Outcomes:
Upon course completion, a student will be able to:

Follow SOPs and perform GLP documentation.
Prepare solutions relevant to protein laboratory work.
Perform accurate and precise volumetric and gravimetric measurements such as micropipetting and weighing.
Use a pH meter and UV-Vis spectrophotometer.
Demonstrate basic protein techniques such as Bradford and BCA assays, SDS-PAGE and column chromatography.

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